Advances in Optical Nanoscopy: From Proof-of-concept to Application
Joerg Bewersdorf
Department of Cell Biology, Department of Biomedical Engineering and Kavli Institute for Neuroscience
Yale University School of Medicine
时间： 2013.10.18 14:00——16：00
地点：玉泉校区教三 326
Abstract：In this presentation, I will provide an overview over current developments in my group in STED microscopy and PALM/STORM, two of the most prominent nanoscopy techniques. I will present the combination of STED microscopy with total internal reflection (TIRF) microscopy which limits excitation to a vicinity of about 100 nm to the cover slip and reduces bleaching and photo damage in live cell imaging [2]. Furthermore, I will describe our latest results in 3D STED microscopy of scattering specimens enabled by the integration of adaptive optics into a custom STED microscope [3].
Additionally, I will present our latest achievement in PALM/STORM where we have recently dramatically improved the recording speed by developing new algorithms which allow the use of new sCMOS cameras for quantitative imaging [4]. In combination with multi-emitter fitting algorithms, we have achieved PALM/STORM imaging of live cells at speeds ranging from 2 to 30 frames per seconds.
Joerg Bewersdorf earned his doctoral degree at the Max Planck Institute for Biophysical Chemistry in Goettingen, Germany, in 2002 under the mentorship of Dr. Stefan Hell, a pioneer in super-resolution microscopy. After a 4-year research appointment at The Jackson Laboratory in Maine, USA, he has joined Yale University School of Medicine as an Assistant Professor.
His research focuses on the development and application of new fluorescence microscopy techniques for biomedical research. Over the last 14 years, he has significantly contributed to the development of multiphoton microscopy (first multifocal multiphoton microscope) and different areas of super-resolution microscopy (4Pi microscopy, PALM/STORM, STED microscopy).
Dr. Bewersdorf has served as the Co-chair of the Nanoscale Biophysics Subgroup of the Biophysical Society of America and is co-founder of a start-up company developing super-resolution microscopes. He is Associate Editor for Optical Nanoscopy and Frontiers in Biophysics and acts as a co-organizer of the yearly Yale Microscopy Workshop.
